The end of AC2, everything during and after the talking with Minerva. I said "What the ****?!" at almost the exact same time as Desmond.
And also in AC2, doing The Truth videos. They were legitimately creepy, and the one where 16 talks about going to the opera will always be one of the most freaky / scary moments I have ever had in video games.
So how about you guys?
Marceline, MY Vampire Queen --> http://tinyurl.com/bzlf5fo