Nope. It sucks as I bought the Pro controller specifically for co-op play and found out it won't work with NSMBU or Nintendoland. The only way to play co-op is to have Wii remotes for additional players.
The Wii U Pro controller even says it will work with most Wii U titles, but definitely not these two.
I wish I could use mine... it's a pain to turn the brightness on the gamepad all the way down so it doesn't shine in my face while I play in a dimly lit room. I refuse to play with the wiimote since that's about as uncomfortable as you can get while playing a videogame imo.
Whether you think you can or can not the answer is yes.
I know it's this consumers fault for not doing the proper research (apparently). But I bought 3 pro controllers for use with this game 4 player. So I pretty much wasted $160 in controllers for no reason.
Way to go Nintendo. You can only use the original Wii remote, even though it doesn't use any motion controls and it would be perfectly easy to play with the pro controller.