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No. You can obtain these costumes by completing all 180 challenges in the Web of Destiny. I used the codes, simply because I was itching to use the Iron Spidey costume right off the bat. I'm still trying to complete all 180 challenges though, simply for the challenge.
To be clear, you unlock Armored-Spider and all the other game's costumes by attaining challenge points and purchasing them, but you unlock the other three pre-order costumes (Negative Zone, Iron-Spider, and Scarlet Spider) by completing the Web of Destiny?
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^Correct. The 3 costumes in question (Iron, Scarlet, Negative AND Mangaverse Spidey as the 4th) are available for purchase AFTER you complete all 180 challenges in the Web of Destiny. The only other way to obtain them is to enter the cheat codes from this site. (No code exists yet for the Mangaverse costume that I know of)
These costumes are considered the ultimate reward in the game which explains why all the other costumes can be obtained much earlier. I personally used the codes but will no doubt complete all 180 challenges anyway as I am trying to get the platinum trophy for this game.
Unlock Iron Spider Costume Up, Right, Right, Right, Left, Left, Left, Down, Up Unlock Scarlet Spider Costume Right, Up, Left, Right, Up, Left, Right, Up, Left, Right Unlocks Negative Zone Suit for use in Noir Levels Left, Right, Right, Down, Right, Down, Up, Left
All codes must be entered at the main menu AFTER you have completed the tutorial.